Chick Enhancer

Poultry-raising is one of the organizations that can be found here in the Philippines. It’s straightforward and accommodating, as chickens give nourishments like chicken meat and eggs. It could likewise help in the horticulture region. A few people utilize supplemental feeds with the goal that the chickens may develop quick. In any case, presently, we’re going to have an examination to locate a characteristic and better substitute, similar to Aloe Vera (Aloe barbadensis) and Malunggay (Moringa oleifera) as added substances in making supplemental feeds. Aloe vera is a delicious plant animal types that likely started in northern Africa. The species doesn't have any normally happening populaces, albeit firmly related aloes do happen in northern Africa. The species is as often as possible refered to as being utilized in natural medication since the start of the main century AD. Concentrates from A. vera are generally utilized in the beautifiers and elective medication ventures, being promoted as differently having reviving, mending, or mitigating properties. There is, in any case, minimal logical proof of the adequacy or security of Aloe vera removes for either restorative or therapeutic purposes, and what positive proof is accessible is oftentimes negated by different investigations. Aloe vera is one of the main realized normal vegan wellsprings of Vitamin B12, and it contains numerous minerals crucial to the development procedure and sound capacity of all the body's frameworks. Moringa oleifera is the most broadly developed types of the class Moringa, which is the main variety in the family Moringaceae. In creating nations, moringa can possibly improve nourishment, help food security, encourage country advancement, and bolster economical landcare. It might be utilized as scavenge for animals, a micronutrient fluid, a characteristic anthelmintic and conceivable adjuvant. Supplemental Feeds, one of the least expensive and most helpful approaches to improve the development of chickens. Articulation of the Problem: This examination plans to decide the impacts of Aloe Vera and Malunggay as added substance parts in making supplemental feeds. The specialists might want to look for the response to the issue â€Å"Can Aloe Vera and Malunggay separate be an option for development and weight enhancer on chicks? † If in this way, what is/are the symptoms of Aloe Vera (Aloe barbadensis) and Malunggay (Moringa oleifera) remove in: * Growth Skin Color * Other attributes Hypothesis: Ho: Aloe Vera and Malunggay extricates are not valuable as added substances in making supplemental feeds. Ha: Aloe Vera and Malunggay separates are viable for utilizing it as added substance segments in making supplemental feeds. Importance of the Study: There is an expansion of interest of chicken today. A large portion of the eateries in our nation are utilizing chicken for the ir claim to fame. As we as a whole realize chicken is one of the most widely recognized nourishments that we eat, and that is our explanation of seeking after this task. We might want to help the poultry-raisers to bring chickens up in a little timeframe. On the off chance that we prevailing with this undertaking we can likewise assist our economy with growing and make it progressively persuasive. This venture is additionally against on non-natural raisers since we don't know about the symptoms of that compound once we admission the chicken. We need to demonstrate that even we are an understudy we can help in our own little manner. The way that Aloe Vera and Malunggay are profoundly accessible and less expensive, this will help individuals, particularly in business ventures. They can even do it all alone.

How to Do a Chain Analysis for Problem Behaviors

How to Do a Chain Analysis for Problem Behaviors PTSD Coping Print How to Do a Chain Analysis to Change Problem Behaviors By Matthew Tull, PhD twitter Matthew Tull, PhD is a professor of psychology at the University of Toledo, specializing in post-traumatic stress disorder. Learn about our editorial policy Matthew Tull, PhD Medically reviewed by Medically reviewed by Daniel B. Block, MD on November 25, 2019 twitter linkedin Daniel B. Block, MD, is an award-winning, board-certified psychiatrist who operates a private practice in Pennsylvania. Learn about our Medical Review Board Daniel B. Block, MD on November 25, 2019 Post-Traumatic Stress Disorder Overview Symptoms & Diagnosis Causes & Risk Factors Treatment Living With In Children Kris Ubach and Quim Roser / Getty Images It can be important for a person with PTSD to learn how to do a chain analysis. People with PTSD  can develop a number of problem behaviors. However it is important to recognize that these problem behaviors develop for a reason. They are serving some kind of function, oftentimes helping someone avoid or escape distress. What Is Chain Analysis? Also known as functional analysis, a  chain analysis is a technique designed to help a person understand the function of a particular behavior.?? During a chain analysis of a particular problem behavior (for example,  deliberate self-harm),?? a person tries to uncover all the factors that led up to that behavior. In other words, a person tries to discover all the links in the chain that ultimately resulted in problem behavior. Therefore a chain analysis  will help you figure out all the things that can contribute to problem behavior. In doing so, a chain analysis can give you insight into how to change such behavior. For example, a person may identify the situation he was in, the thoughts he was experiencing, or the feelings he was having just prior to engaging in that behavior. In doing so, a person can increase his awareness of all the factors that may put him at risk for problem behavior. This way a person has better ability to intervene early on to prevent that behavior in the future. Identify What You Want to Change The first step is to identify the behavior you want to change. For example, do you want to stop engaging in self-medication through alcohol? Binge eating? Try to identify a behavior that is causing problems for you in your life. Next, think about what happened prior to you engaging in the problem behavior. What were you doing? What was going on around you? Were you in an argument? Did you have a memory of your traumatic event triggered? Basically, you want to identify the event or situation that served as the starting point for your problem behavior. 4 Steps to Shift Perspective and Change Everything Pay Attention to Thought Patterns and Feelings Now, identify what kinds of thoughts were brought up by the situation or event that led to the problem behavior. How did you evaluate the situation or yourself in that situation? Did you engage in catastrophic or all-or-none thinking? Think about what emotions you were having as a result of that situation. Try your best to list as many emotions as you possibly can, such as fear, sadness, anger, shame, guilt, embarrassment, or dread. Pay attention to what you felt in your body. Try to recognize and label all the sensations that came up. For example, did you experience shortness of breath? Muscle tension? An increased heart rate? Think about how your body reacted to the situation. Next, list off what your thoughts, emotions and bodily sensations made you want to do. That is, did they make you want to escape the situation or do something to make those feelings stop? Did you feel a need to engage in your problem behavior? Finally, think about the consequences of engaging in your problem behavior. Did you feel better afterward? Did you feel disappointed in yourself? Ashamed? Try to list as many consequences (both positive and negative) as you can. Using Self-Monitoring for PTSD to Bring Awareness of Your Emotions Tips It can be helpful to go through a chain analysis soon after you engage in problem behavior. This way, your experience is fresh in your mind and you will likely be able to remember more information about the factors that led up to your problem behavior. It might also be helpful to identify what things might have made you more susceptible to responding to the situation as you did. For example, when people do not eat well or do not get enough sleep, they may be more susceptible to experiencing negative moods or having more reactive emotional experiences. Behaviors can serve multiple functions. Therefore, go through a chain analysis for a number of different situations that led to a problem behavior and try to identify all the functions a problem behavior serves for you. After you go through the chain analysis, come up with different coping strategies you could use at each stage. In addition to identifying the function a problem behavior serves, it is also incredibly important to figure out how to break the chain through the use of healthier coping strategies.?? PTSD: Coping, Support, and Living Well

Management Can Be Seen As An Art - 1469 Words

On the other hand it could be argued that management can be seen as an art. Literature proves the Armed Forces leaders to be the best at managing change â€Å"Orion claims the most effective approach to huge cuts and organisational upheaval is for leaders to persuade their staff to understand why changes are good for them, a process that involves asking employees lots of questions about how they are feeling. This kind of brain-friendly leadership is more prevalent in the armed forces† (Dobinson, 2012). This in itself is an art form as the Armed Forces leaders are taking practical knowledge and skill and applying it to their subordinates. It is arguable that since managers are accountable for the smooth running of new strategies, management can be perceived as an art. This is due to organisational change requiring communication, vision and craft to avoid crisis. â€Å"Leaders of successful defence transformations resist the urge to reorganise; they focus first on securing the successes that can make a big difference to the momentum of a program† (Chinn Dowdy, 2014). This in itself is an art form as leaders are improving performance and altering knowledge into actions to reach measurable results. The knowing-doing gap by Pfeffer and Sutton alongside creative action articulates that a business that acts upon their knowledge eliminates fear, eradicates destructive internal competition. Managers must have the appropriate tools to accomplish their tasks and have a vision to employShow MoreRelatedThe City Upkeep And Maintenance Of Art Preservation Essay1332 Words   |  6 Pagesand a collection of arts. With developments untaken by the city council that which makes Lawrence uniquely is under threat, due to the changing landscape around the community. While this is a changing landscape questions arise of how the arts are being implicated in this city landscape and the future of artistic development. This research will focus on the city upkeep and maint enance of art preservation and the roles which Lawrence plays as well as the benefits of proper arts interjection. Read MoreEssay about Exploring the Arts and Resource Management1369 Words   |  6 PagesThe term â€Å"resource management† is an umbrella term that encompasses all forms of resources and how they can be developed and managed to create an effective and efficient use of the organizations available resources. Resources are typically thought of as including tangible things such as goods, buildings, equipment, technology, financial resources, and people, or non-tangible things like creative ideas, vision, and mission. 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I have hig h standards, but I am open to letting others find better way to complete a task. If they fail, I will take over and do the task myself, or let someone I find more knowledgable do it. I usually work byRead MoreDatabase Analysis : The Metropolitan Museum Of Art878 Words   |  4 Pages storage, management, and use of data within a database environment†. (Database Systems, 2013) A database is part of a larger system known as an information system, it helps with the collection, storage and retrieval of data. The database structure needs to be defined and its fields labeled properly for it to be an asset to the user. The database environment I have selected to analyze is the Metropolitan Museum of Art in New York City. On April 13, 1870, the Metropolitan Museum of Art was opened

Customer Satisfaction - Framework - 9815 Words

Customer Satisfaction IMPROVING QUALITY AND ACCESS TO SERVICES AND SUPPORTS IN VULNERABLE NEIGHBORHOODS Customer Satisfaction Customer Satisfaction IMPROVING QUALITY AND ACCESS TO SERVICES AND SUPPORTS IN VULNERABLE NEIGHBORHOODS February 2007 TABLE OF CONTENTS I. Introduction ................................................................................................................... 7 II. What will a Customer Satisfaction Framework Offer ........................... 9 A. A Hypothetical Example — Application to Public Human Services ......................... 9 B. A Hypothetical Example of an Engaged and Informed Resident Consumer Strategy†¦show more content†¦Improving service quality to meet customers’ standards is an ongoing part of doing business. In this way, customers drive the market and the organization. At the same time that organizations act to attract and satisfy customers, the customers themselves exercise ultimate influence. Their satisfaction depends on both their expectations and their treatment. Through their choices, customers decide which organizations survive and thrive, determine what goods and services are available, and shape how they are provided. In addition to using their purchasing power, informed consumers can shape the marketplace by communicating their preferences and standards to organizations that are poised to listen and respond. Contents of This Paper For both service providers and consumers, making customer satisfaction a priority involves a fundamental shift in thinking, organizing and acting. The customer satisfaction framework proposed in this paper explores the potential of a customer satisfaction orientation as a means for improving the goods and services, including those provided by the public sector, available to residents of tough neighborhoods. 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Civil Rights Movement Free Essays

CIVIL RIGHTS MOVEMENT The civil rights movement in America had a wide variety of successes over time. As successful as they were, all went through their share of hardships and struggles. Major desegregation acts in history include Brown vs, Board of Education, the Montgomery Bus Boycott, and the Civil Rights Act of 1957. We will write a custom essay sample on Civil Rights Movement or any similar topic only for you Order Now These events changed life for African Americans to come. In the early 1950’s, racial segregation in public schools was the norm across America. Although all the schools in a given district were supposed to be equal, most black schools were inferior to their white counterparts. Brown vs. Board of Education was a decision of the US Supreme Court in 1954 that declared state laws establishing separate public schools for black and white students was unconstitutional. It was a giant step towards complete desegregation of public schools. However, even partial desegregation of these schools, was still very far away. Started by the arrest of Rosa Parks on 1 December 1955, the Montgomery bus boycott was a 13-month protest that ended with the U. S. Supreme Court ruling that segregation on public buses is unconstitutional. At one point in time, 90 percent of African American bus riders were choosing to walk. The bus boycott demonstrated nonviolent protest to successfully challenge racial segregation and was an example for other campaigns that followed. Dr. Martin Luther King Jr. , being the president of the Montgomery Improvement Association, spoke to many about the Montgomery Bus Boycott, commonly expressing that, ‘‘I want it to be known that we’re going to work with grim and bold determination to gain justice on the buses in this city†. The bus boycott was followed by a similar judgment concerning interstate buses. However, states in the Deep South continued their own policy of transport segregation. The 1964 Civil Rights Act made racial discrimination in public places, such as theaters, restaurants and hotels, illegal. It also required employers to give equal employment opportunities. The Civil Rights Act also attempted to deal with the problem of African Americans being denied the vote in the Deep South. The legislation stated it must prevail for establishing the right to vote. Schooling to sixth grade constituted legal proof of literacy and the attorney general was given power to give legal action in any area that they found resistance to the law. These three things impacted American history and all strengthened a complete integration that many during this time were moving toward. Without these, who knows where the U. S. would be at, in terms of racial issues, today? How to cite Civil Rights Movement, Papers Civil Rights Movement Free Essays During the 1960s it was the start of the civil rights movement. It’s quite a significant period in American history for equality since the Civil war. African American has had enough of the unjust way they’ve been treated. We will write a custom essay sample on Civil Rights Movement or any similar topic only for you Order Now This was about the fight to attain something more than just changing the law but also come together as a nation to continue the fight for freedom and solve these social issues that were oppressing people of color. In order for this to happen, African Americans must of have taken actions that would greatly affect the system that they were forced to live. This was a time where uniting in their community was essential to the cause of the civil rights movement. Overcoming all these challenges was difficult as they were met with many oppositionists that were peaceful and non-peaceful. Many different leaders had a different philosophy on how to resolve their issues. Ultimately, they all had the same goal which was to find freedom and equality for their people. The civil rights movement was about battling against the racial injustices in America and getting equality to all people, while at the same time offering solutions to resolve all these issues in America. In the civil rights movement, African American were faced with dangers and obstacles in this fight. With all the rallies, marches that were organized, or just simply living their life, came a lot of unwelcome physical violence. One prime example of this is the peaceful marches people came and were then beaten by the police. Civil rights leader Martin Luther King, Jr. directly explains, â€Å"when you have seen hate-filled policemen curse, kick, and even kill your black brothers and sisters† (p.28-29).Most of the time it was no fault of the protester, but it was because of the racism and hate police had people of color. Another, yet similar, example of violence, experienced by Anne Moody. This was a protest in the death of Medgar. Anne, as well as other protesters, were arrested and put into a paddy wagon or garbage trucks and left in the fields. â€Å"The driver rolled up the windows,† describes civil rights activist Anne Moody, â€Å"turned the heater on, got out, closed the door and left us† (p.49). The protesters were not treated with any respect but instead treated like animals. Police brutality was a reality that was expected to be confronted with at rallies. However, they had to show no signs no signs of counter-attack and peaceful throughout the protest. It was part of King’s non-violent approach during the civil rights movement. The oppression and discrimination of sorts was a battle that people of color faced during the 1960s. All of this was part of an everyday life for African Americans. However, there was some type discrimination within them. This was the role that women were allowed to be part of in the marches. Civil rights activist Rosa Parks states, â€Å"women were not allowed to play much of a role. The marching committee didn’t want Coretta Scott King and the other wives of the male leaders to march with their husbands† (p.44). This was the separation of women and men for no reason. It shows the inequality that was present during the 1960s but also a bit ironic since the whole point of the march was the fight for equal rights. It’s also a representation of how much more work is needed to make this world equal for everyone. Moreover, the discrimination against color people was quite vibrant in all aspects of the ways African Americans lived. African Americans were hit with a lot of discriminatory signs in public accommodations that were extremely racially offensive. When there seemed to have been some type of change with that, it only lasted for a while and went back to the same old thing. â€Å"We realize that we were the victims of a broken promise. A few signs, briefly removed, returned; the others remained,† expresses activist Martin Luther King, Jr. (p.26). Just because of their skin color they were met with racist signs. As I said before it’s not just a legal change that was occurring but also a social one that needed a change. It was important to have a change in opinion by this country’s society. It was also about combating the racism black people faced on a daily basis throughout their day. Anne Moody was a victim of what black people faced and hoped to change. Anne Moody was physically abused because of the color of her skin, â€Å"then the mob started to smear her and others with ketchup, mustard, sugar, pies, and everything in the counter† (p.45). This was just for sitting at the counter in a restaurant. In the midst of all this chaos, African Americans took action to combat all the racism they were facing and unjust laws that in order to get to a solution. There were many strategies that civil rights activist proposed and practiced that were put on the table in order to find solutions. One of these influencers proposed to have a unification of all black people. â€Å"Keep our religion between ourselves and our God, but when coming out here, we have a fight that is common to all of us against an enemy who is common to all of us,† expresses Muslim and civil rights leader Malcolm X (p.72-73). This a time where everyone must put their beliefs and differences aside in order to progress. At the end of the day, they all have the same goal and the same foe to fight against. This is a call to action where they must gain control of their own fate but in order for that to happen, they must invest in their own self. One of Malcolm’s main objectives to combat the oppressive system black people were living in was different to King’s method. King’s method was a non-violent one and have all people, no matter the color, come together to erase the racism between people. Malcolm was more having only black people come together as a community to help one another in order to progress. Muslim speaker states, â€Å"The community in which you spend your money becomes richer and richer. The community out of which you take your money becomes poorer and poorer† (p.74). Malcolm wants black people to stop spending their money in communities they are not part of. He insists that it by spending money in your own community, it becomes richer and in then being able to prosper. This was just one of the few things that he proposes to during this time period. The 1960s was another major time period for African Americans to fight for equal rights in this country. This time period is a big turning point for all people of color. It signifies that by uniting everyone for a cause, situations can be changed for the better no matter how difficult the obstacle. It was the fight to change the social, political, cultural, and laws that cast a shadow on the people of color by discriminating them. The civil rights movement gave birth to different many leaders on how to solve this issues that were happening at that time. With many people taking action and finding solutions, also came a lot of misfortunes that brought many difficulties in the fight for equality. Many discrimination acts were against African American that they had the courage to unite for a better tomorrow. It was all about getting equality for every single person. Although today our world is not completely equal as we want it to be, there is no doubt that we have made some remarkable advancements in our society. Looking back at the history of the civil rights movement there’s for sure something that we learn from and use today to solve our current issues. How to cite Civil Rights Movement, Papers Civil Rights Movement Free Essays The success achieved following the years of the Second World War only determined the Civil Rights activists to continue their fight for equal treatment. The important Supreme Court ruling of Brown v The Board of Education outlawed the segregated state sponsored school system, which had promoted legal segregation of elementary schools. However there was much need for a proper implementation of this decision and for further legal action. We will write a custom essay sample on Civil Rights Movement or any similar topic only for you Order Now The murder of Emmet Till and the subsequent acquittal of his white killers represented a lost opportunity for the justice system to lay its impartial role. From there on, the goals, the leaders and the tactics of the Civil Rights movement changed from legal to direct actions. This evolution was partly due to the fact that there were continuous attempts to interfere with legal actions that the NAACP was undergoing in achieving equality for the Black community. Thus the main goal became now the determined fight against segregation with clear targets such as desegregation of Albany or Birmingham. (Jenkins 1997). The means however, although they was less legal action involved, remained non-violent, and often took the form of boycotts, freedom rides. One such example was the Montgomery Bus Boycott against the segregated transportation system in Alabama, which resulted in the end in a Supreme Court ruling against the State of Alabama. The tactics involved activities at the local level, which were now conducted by Church members, thus the community became much more implicated. Among these, Dr. Martin Luther King, Jr. who would later become one of the leading figures of the Civil Rights Movement. Also, sit-ins were organized by students in order to encourage the desegregation in schools. The efforts culminated with the Voting Rights Act of 1965. (African American Odyssey 2002). However, slowly, the cooperation between the black civil rights militants and the whites became an obstacle for those who believed in more radical moves. Thus leaders such as Stokely Carmichael began advocating a new concept, that of â€Å"black power† which demanded freedom from white authority both economically and political. Its means of representation sought more an improvement of the black communities rather than their integration in the white reality. Thus, it encouraged self consciousness and self reliance, along with the creation of a strong cultural conscience. The role of the black women is rather important as they too strived for recognition of their rights. However, the emancipation movement that had galvanized the black community was doubled by the feminist movement which in its turn demanded for equal rights for men and women. (Williams n. d. ). Nonetheless, from a wider perspective, black women represented an indispensable element for the complete emancipation of the black community in offering both practical and moral support. One such personality was Gospel Singer Mahalia Jackson who had joined the Civil Rights Movement at the request of Dr. King and who represented an important figure for the black cultural emerging identity. (African American Odyssey 2002). Bibliography African American Odyssey. (2002). Sit-ins, Freedom Rides, and Demonstrations. Retrieved 9 May 2006 from Library of Congress Web site: http://memory. loc. gov/ammem/aaohtml/exhibit/aopart9b. html Jenkins, P. (1997). A history of the United States. New York: Palgrave. Williams, M. (n. d). Black Women and the Struggle for Liberation. [Electronic version]. Third World Women’s Alliance. Black Women’s Manifesto. NY: Third World Women’s Alliance. How to cite Civil Rights Movement, Papers

Architectural Branding free essay sample

The 4P’s have been extended to 7P’s namely: Price, Place, Promotion, People, Physical Evidence and Process. Architectural Branding is an important element of Physical Evidence very strongly prevailing in International Brands e. g. Calvin Klein, Nike town, DG, Prada etc. With the changing Indian Market scenario and customer awareness Indian Brands have now realised to incorporate the culture of Architectural Branding as an important brand element. Possibly Brands like Colour Plus, Wills Lifestyle, Sepia also are trying to incorporate the concept of Architectural Banding. Therefore through an exploratory research on the related topics of branding it was felt that architectural branding will help us in the long run in the industry and would widen our horizon of knowledge. Down the line I felt that this concept would be taught to us in our course curriculum of Fashion Management. Through this topic of Architectural Branding we would get to learn about basics of Architecture, Interior Design and Design Space and relating these subjects to the Market Research and Consumer Behaviour. We will write a custom essay sample on Architectural Branding or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page Information Needed ? Introduction to Architectural Branding Concepts of Architecture †¢ Material †¢ Types of Structure ? Architecture in Retail †¢ Exteriors †¢ Interior Design †¢ Store Layout, Design

Guava fruit Essay Example

Guava fruit Essay The intestinal digestive enzymes alpha-glucosidase and alpha-amylase are plays a vital role in the carbohydrate digestion. One antidiabetic therapeutic approach reduces the post prandial glucose level in blood by the inhibition of alpha-glucosidase and alpha-amylase enzymes. These can be an important strategy in management of blood glucose. The aim of the present study was to investigate the phytochemical bioactive compounds of the methanolic extract of Psidium guajava leaves, its in vitro anti-diabetic activity.The assay results suggests that the presence of bioactive compounds, could be responsible for the versatile medicinal properties of this plant including diabetes, the extract exhibit the dose-dependent increase in inhibitory effect on alpha-glucosidase enzyme (upto 89. 4%), and alpha-amylase enzyme (upto 96. 3%). The current study proves that the antidiabetic activity of methanolic extract of Psidium guajava leaves by in vitro studies. * Keywords Psidium guajava; Phytochemica l; alphaglucosidase; alpha-amylase Introduction Diabetes mellitus is a complex and a diverse group of disorders that disturbs he metabolism of carbohydrate, fat and protein. The number of diabetes mellitus cases has been increasing worldwide in recent years. In 2000, the world health organization estimated a total of 171 million of people with diabetes mellitus from the global population, and this report projected to increase to 366 million by 2030 (Wild et al. , 2004). With a long course and serious complications often resulting in high deathrate, the treatment of diabetes spent vast amount of resources including medicines, diets, physical training and so on in all countries.Thus searching for a new class of compounds is essential to overcome diabetic problems. There is continuous search for alternative drugs (Syamsudin, 2010). 15 Int. J. Curr. Microbiol. App. Sci (2013) 2(2):15-19 Psidium guajava Linn. (Myrtaceae) is commonly known as Peru or Guava in English. The Psidium guajava leaves are used in the treatment of diarrhoea, cough, stomachache, dysentery and decoction of the leaves for cholera patients, toothache and gum boils. The leaves showed hypoglycemic, cardioprotective, myocardial depressant, antimicrobial, antispasmodic actions (Ross, 1999).Inhibition of alphaamylase and alpha-glucosidase enzymes can be an important strategy in management of post prandial blood glucose level in type 2 diabetes patient (Ali et al. , 2006). Thus, objective of the present study is to investigate the phyto chemical, invitro antidiabetic activity of methanolic extract of Psidium guajava leaves. reducing sugar, tannins, flavonoids, steroids and alkaloids by using standard phytochemical procedures (Evans, W. C and Evans, T. 2003). In vitro methods employed in antidiabetic studies Inhibition of alpha-amylase enzyme A starch solution (0. 1% w/v) was obtained by stirring 0. g of potato starch in 100 ml of 16 mM of sodium acetate buffer. The enzyme solution was prepared by mix ing 27. 5 mg of alpha-amylase in 100 ml of distilled water. The colorimetric reagent is prepared by mixing sodium potassium tartarate solution and 3, 5 di nitro salicylic acid solution 96 mM. Both control and plant extracts were added with starch solution and left to react with alpha- amylase solution under alkaline conditions at 25? C. The reaction was measured over 3 minutes. The generation of maltose was quantified by the reduction of 3, 5 dinitro salicylic acid to 3amino-5- nitro salicylic acid.This reaction is detectable at 540 nm (Malik and Singh, 1980). Materials and Methods Plant material and extraction The fresh leaves of Psidium guajava were collected locally and authenticated by the department of Botany, St. Joseph College, Trichy. The shade dried Psidium guajava leaves were powdered mechanically and stored in an air tight container. The extraction was carried out by hot percolation method using Soxhlet apparatus. The solvent used was methanol. About 100 gm of powder was extracted with 600 ml of methanol. The extract was concentrated to dryness under controlled temperature 4050Â °C.The percentage yield was found to be 10. 15%. The extract was preserved in refrigerator till further use. Phytochemical Screening For preliminary phytochemical analysis the freshly prepared crude methanolic extracts of leaves were tested for the presence or absence of phytoconstituents such as 16 Inhibition of alpha-glucosidase enzyme The inhibitory activity was determined by incubating a solution of starch substrate (2 % w/v maltose or sucrose) 1 ml with 0. 2 M Tris buffer pH 8. 0 and various concentration of plant extract for 5 min at 37Â °C.The reaction was initiated by adding 1 ml of alpha-glucosidase enzyme (1U/ml) to it followed by incubation for 40 min at 35Â °C. Then the reaction was terminated by the addition of 2 ml of 6N HCl. Then the intensity of the colour was measured at 540nm (Krishnaveni et al. , 1984). Int. J. Curr. Microbiol. App. Sci (2013) 2(2):15-1 9 Calculation of 50% Inhibitory Concentration (IC50) The concentration of the plant extracts required to scavenge 50% of the radicals (IC50) was calculated by using the percentage scavenging activities at five different concentrations of the extract.Percentage inhibition (I %) was calculated by I % = (Ac-As)/Ac X 100, (Shai et al. , 2010). where Ac is the absorbance of the control and As is the absorbance of the sample. Table 1 Phytochemical analysis of methanolic extract of Psidium guajava leaves S. No. Phytochemical Constituents Name of the Test Mayer s test Dragondraff test Wagner Test Molish Test Fehling Test Benedicts Test Lead Acetate Ferric chloride. Ammonia Salkowaski H2So4 Liebermann s Burchard Test H2So4 Ammonia Shinoda s Test Ferric chloride Sodium chloride Borntrager s test Methanolic Extract + + + + + + + Condensed Tannin + + + + + _ Alkaloids 2 3 4 5 6 Carbohydrates Tannins Pseudo tannins Chlorogenic acid Steroidal Glycosides Anthocyanin Steroidal Glycosides Saponins g lycosides Flavonoids Flavones Phenols Coumarin Phytochemical constituents Results The preliminary phytochemical screening tests for the methanol extract of Psidium guajava leaves (Table 1) revealed the presence of carbohydrates, alkaloids, flavones, tannins, steroidal glycosides, phenols and coumarin. Any of these secondary metabolites, singly or in combination with others could be responsible for the anti-diabetic activity of the plant.There was a dose-dependent increase in percentage inhibitory activity against alphaamylase enzyme. At a concentration of 0. 2 ml of plant extract showed a percentage inhibition 27. 8% and for 1. 0 ml plant extract showed inhibition of 96. 3% (Table 2). The Psidium guajava methanol extract revealed a significant inhibitory action of alpha-glucosidase enzyme. The percentage inhibition at 0. 2-1. 0 ml concentrations of Psidium guajava extract showed a dose dependent increase in percentage inhibition. The percentage inhibition varied from 89. 4% 31. 7% for highest concentration to the lowest oncentration (Table 3). 7 8 9 10 11 12 13 14 +++= High; + = Present; ++ = Moderate; = Absent Table. 2 In vitro antidiabetic activity of alpha-amylase method S. No 1 2 3 4 5 Concentration of Sample (ml) 0. 2 0. 4 0. 6 0. 8 1. 0 % of Inhibition 27. 8 48. 9 62. 5 87. 2 96. 3 17 Int. J. Curr. Microbiol. App. Sci (2013) 2(2):15-19 Table 3 In vitro antidiabetic activity of alpha glucosidase method S. No 1 2 3 4 5 Concentration of Sample (ml) 0. 2 0. 4 0. 6 0. 8 1. 0 % Of Inhibition 31. 7 53. 9 68. 2 76. 5 89. 4 Discussion Diabetes mellitus is a metabolic disorder with increasing incidence throughout the world.Insulin is a key player in the control of glucose homeostasis. Lack of insulin affects carbohydrate, fat and protein metabolism (Rajiv Gandhi and Sasikumar, 2012). Management of diabetes without side effects is still challenge to the medical community. It was proposed that inhibition of the activity of such alpha-amylase and alpha-glucosidase would delay the degradation of carbohydrate, which would in turn cause a decrease in the absorption of glucose, as a result the reduction of postprandial blood glucose level elevation (Rhabaso Lhoret and Chiasson, 2004).In the present study, research has been carried out to evaluate the preliminary phytochemical investigation and the potential of methanol extract of Psidium guajava leaf in inhibiting alpha-glucosidase and alpha-amylase. The present finding of Phytochemical screening of the plant extract confirmed the presence of several bioactive compounds like alkaloids, flavones, tannins and phenols which could be responsible for the versatile medicinal properties of this plant. The present finding reveals that Psidium guajava efficiently inhibits both alpha18 mylase and alpha-glucosidase enzymes in vitro in a dose dependent manner. The aqueous extracts from Syzygium cumini seeds and Psidium guajava leaves both showed a dose dependent inhibitory effect on alpha-amylase activity (K arthic et al. , 2008). The antidiabetic action of Psidium guajava can also be attributed to the intestinal alpha-amylase and alphaglucosidase inhibitory activity. Further studies are required to elucidate whether Psidium guajava have antidiabetic potential by in vivo for validating the traditional claim of the plant.In this present study we evaluated in vitro alpha amylase and alpha glucosidase activity of crude methanol extract of Psidium guajava leaves. The plant showed significant inhibition activity, so further the compound isolation, purification and characterization which is responsible for inhibiting activity, has to be done for the usage of antidiabetic agent. Acknowledgement The authors are grateful to Dr. P. Selvaraj, Chairman, Shivani Group of Institutions, Trichy, whose spacious heart cheered our efforts to process this venture properly, successfully to complete the task.Also we extend our thanks to The Secretary, The Principal and The Staff members of the SSK Polytechni c College, Trichy, Tamilnadu, India. References Ali, H. , P. J. Houghton, and Soumyanath, A. 2006. J. Ethnopharmacol. 107: 449-455. Evans, W. C. , and Evans, T. 2003. Pharmacognosy, 5th Edn. , Cambridge University Press, London, 336-93. Int. J. Curr. Microbiol. App. Sci (2013) 2(2):15-19 Karthic, K. , K. S. Kirthiram, S. Sadasivam, and Thayumanavan, B. 2008. Identification of amylase inhibitors from Syzygium cumini Linn seeds. Indian J. Exp. Biol. 6:677- 680. Krishnaveni,S. , B.Theymoli, and Sadasivam,S. 1984. Food Chem. 15: 229. Malik,C. P. , and Singh, M. B. 1980. Plant Enzymology and Histoenzymology, Kalyani Publishers, New Delhi, p. 278. Rajiv Gandhi, G. , and Sasikumar, P. 2012. Antidiabetic effect of Merremia emarginata Burm. F. in Streptozotocin induced diabetic rats. Asian. Paci. J. Tropi. Biomedicine. 2: 281-286. Rhabaso Lhoret, R. , and Chiasson, J. L. , 2004. Glucosidase inhibitors. In: Defronzo R. A, Ferrannini E, Keen H, Zimmet P. (Eds. ), International Textbook of Di abetes Mellitus, Vol. 1, third ed. John Wiley and Sons Ltd. , UK, pp. 901-914. Ross, I. A. 1999. Medicinal Plants of the World, Chemical constituents, traditional and modern medicinal uses. Human Press Inc. , Totowa, NJ. 263-272. Shai, L. J. , P. Masoko, M. PMokgotho, S. R. Magano, M. A. Mogale, N. Boaduo, and Eloff, J. N. 2010. South African J. Bot. 76: 465 470. Syamsudin,S. , 2010. Standardization of extract of Leucaena leucocephala (lmk) De Wit seeds by -glucosidase inhibitor. Int. J. Phytomedicine. 2. 430-435. Wild, S. , G. Roglic, A. Green, R. Sicree, and King, H. 2004. Global prevalence of Diabetes estimates for the year 2000 and projections for 2030. Diabetes care. 27:1047-1053. 19 Guava fruit Essay Example Guava fruit Essay The intestinal digestive enzymes alpha-glucosidase and alpha-amylase are plays a vital role in the carbohydrate digestion. One antidiabetic therapeutic approach reduces the post prandial glucose level in blood by the inhibition of alpha-glucosidase and alpha-amylase enzymes. These can be an important strategy in management of blood glucose. The aim of the present study was to investigate the phytochemical bioactive compounds of the methanolic extract of Psidium guajava leaves, its in vitro anti-diabetic activity.The assay results suggests that the presence of bioactive compounds, could be responsible for the versatile medicinal properties of this plant including diabetes, the extract exhibit the dose-dependent increase in inhibitory effect on alpha-glucosidase enzyme (upto 89. 4%), and alpha-amylase enzyme (upto 96. 3%). The current study proves that the antidiabetic activity of methanolic extract of Psidium guajava leaves by in vitro studies. * Keywords Psidium guajava; Phytochemica l; alphaglucosidase; alpha-amylase Introduction Diabetes mellitus is a complex and a diverse group of disorders that disturbs he metabolism of carbohydrate, fat and protein. The number of diabetes mellitus cases has been increasing worldwide in recent years. In 2000, the world health organization estimated a total of 171 million of people with diabetes mellitus from the global population, and this report projected to increase to 366 million by 2030 (Wild et al. , 2004). With a long course and serious complications often resulting in high deathrate, the treatment of diabetes spent vast amount of resources including medicines, diets, physical training and so on in all countries.Thus searching for a new class of compounds is essential to overcome diabetic problems. There is continuous search for alternative drugs (Syamsudin, 2010). 15 Int. J. Curr. Microbiol. App. Sci (2013) 2(2):15-19 Psidium guajava Linn. (Myrtaceae) is commonly known as Peru or Guava in English. The Psidium guajava leaves are used in the treatment of diarrhoea, cough, stomachache, dysentery and decoction of the leaves for cholera patients, toothache and gum boils. The leaves showed hypoglycemic, cardioprotective, myocardial depressant, antimicrobial, antispasmodic actions (Ross, 1999).Inhibition of alphaamylase and alpha-glucosidase enzymes can be an important strategy in management of post prandial blood glucose level in type 2 diabetes patient (Ali et al. , 2006). Thus, objective of the present study is to investigate the phyto chemical, invitro antidiabetic activity of methanolic extract of Psidium guajava leaves. reducing sugar, tannins, flavonoids, steroids and alkaloids by using standard phytochemical procedures (Evans, W. C and Evans, T. 2003). In vitro methods employed in antidiabetic studies Inhibition of alpha-amylase enzyme A starch solution (0. 1% w/v) was obtained by stirring 0. g of potato starch in 100 ml of 16 mM of sodium acetate buffer. The enzyme solution was prepared by mix ing 27. 5 mg of alpha-amylase in 100 ml of distilled water. The colorimetric reagent is prepared by mixing sodium potassium tartarate solution and 3, 5 di nitro salicylic acid solution 96 mM. Both control and plant extracts were added with starch solution and left to react with alpha- amylase solution under alkaline conditions at 25? C. The reaction was measured over 3 minutes. The generation of maltose was quantified by the reduction of 3, 5 dinitro salicylic acid to 3amino-5- nitro salicylic acid.This reaction is detectable at 540 nm (Malik and Singh, 1980). Materials and Methods Plant material and extraction The fresh leaves of Psidium guajava were collected locally and authenticated by the department of Botany, St. Joseph College, Trichy. The shade dried Psidium guajava leaves were powdered mechanically and stored in an air tight container. The extraction was carried out by hot percolation method using Soxhlet apparatus. The solvent used was methanol. About 100 gm of powder was extracted with 600 ml of methanol. The extract was concentrated to dryness under controlled temperature 4050Â °C.The percentage yield was found to be 10. 15%. The extract was preserved in refrigerator till further use. Phytochemical Screening For preliminary phytochemical analysis the freshly prepared crude methanolic extracts of leaves were tested for the presence or absence of phytoconstituents such as 16 Inhibition of alpha-glucosidase enzyme The inhibitory activity was determined by incubating a solution of starch substrate (2 % w/v maltose or sucrose) 1 ml with 0. 2 M Tris buffer pH 8. 0 and various concentration of plant extract for 5 min at 37Â °C.The reaction was initiated by adding 1 ml of alpha-glucosidase enzyme (1U/ml) to it followed by incubation for 40 min at 35Â °C. Then the reaction was terminated by the addition of 2 ml of 6N HCl. Then the intensity of the colour was measured at 540nm (Krishnaveni et al. , 1984). Int. J. Curr. Microbiol. App. Sci (2013) 2(2):15-1 9 Calculation of 50% Inhibitory Concentration (IC50) The concentration of the plant extracts required to scavenge 50% of the radicals (IC50) was calculated by using the percentage scavenging activities at five different concentrations of the extract.Percentage inhibition (I %) was calculated by I % = (Ac-As)/Ac X 100, (Shai et al. , 2010). where Ac is the absorbance of the control and As is the absorbance of the sample. Table 1 Phytochemical analysis of methanolic extract of Psidium guajava leaves S. No. Phytochemical Constituents Name of the Test Mayer s test Dragondraff test Wagner Test Molish Test Fehling Test Benedicts Test Lead Acetate Ferric chloride. Ammonia Salkowaski H2So4 Liebermann s Burchard Test H2So4 Ammonia Shinoda s Test Ferric chloride Sodium chloride Borntrager s test Methanolic Extract + + + + + + + Condensed Tannin + + + + + _ Alkaloids 2 3 4 5 6 Carbohydrates Tannins Pseudo tannins Chlorogenic acid Steroidal Glycosides Anthocyanin Steroidal Glycosides Saponins g lycosides Flavonoids Flavones Phenols Coumarin Phytochemical constituents Results The preliminary phytochemical screening tests for the methanol extract of Psidium guajava leaves (Table 1) revealed the presence of carbohydrates, alkaloids, flavones, tannins, steroidal glycosides, phenols and coumarin. Any of these secondary metabolites, singly or in combination with others could be responsible for the anti-diabetic activity of the plant.There was a dose-dependent increase in percentage inhibitory activity against alphaamylase enzyme. At a concentration of 0. 2 ml of plant extract showed a percentage inhibition 27. 8% and for 1. 0 ml plant extract showed inhibition of 96. 3% (Table 2). The Psidium guajava methanol extract revealed a significant inhibitory action of alpha-glucosidase enzyme. The percentage inhibition at 0. 2-1. 0 ml concentrations of Psidium guajava extract showed a dose dependent increase in percentage inhibition. The percentage inhibition varied from 89. 4% 31. 7% for highest concentration to the lowest oncentration (Table 3). 7 8 9 10 11 12 13 14 +++= High; + = Present; ++ = Moderate; = Absent Table. 2 In vitro antidiabetic activity of alpha-amylase method S. No 1 2 3 4 5 Concentration of Sample (ml) 0. 2 0. 4 0. 6 0. 8 1. 0 % of Inhibition 27. 8 48. 9 62. 5 87. 2 96. 3 17 Int. J. Curr. Microbiol. App. Sci (2013) 2(2):15-19 Table 3 In vitro antidiabetic activity of alpha glucosidase method S. No 1 2 3 4 5 Concentration of Sample (ml) 0. 2 0. 4 0. 6 0. 8 1. 0 % Of Inhibition 31. 7 53. 9 68. 2 76. 5 89. 4 Discussion Diabetes mellitus is a metabolic disorder with increasing incidence throughout the world.Insulin is a key player in the control of glucose homeostasis. Lack of insulin affects carbohydrate, fat and protein metabolism (Rajiv Gandhi and Sasikumar, 2012). Management of diabetes without side effects is still challenge to the medical community. It was proposed that inhibition of the activity of such alpha-amylase and alpha-glucosidase would delay the degradation of carbohydrate, which would in turn cause a decrease in the absorption of glucose, as a result the reduction of postprandial blood glucose level elevation (Rhabaso Lhoret and Chiasson, 2004).In the present study, research has been carried out to evaluate the preliminary phytochemical investigation and the potential of methanol extract of Psidium guajava leaf in inhibiting alpha-glucosidase and alpha-amylase. The present finding of Phytochemical screening of the plant extract confirmed the presence of several bioactive compounds like alkaloids, flavones, tannins and phenols which could be responsible for the versatile medicinal properties of this plant. The present finding reveals that Psidium guajava efficiently inhibits both alpha18 mylase and alpha-glucosidase enzymes in vitro in a dose dependent manner. The aqueous extracts from Syzygium cumini seeds and Psidium guajava leaves both showed a dose dependent inhibitory effect on alpha-amylase activity (K arthic et al. , 2008). The antidiabetic action of Psidium guajava can also be attributed to the intestinal alpha-amylase and alphaglucosidase inhibitory activity. Further studies are required to elucidate whether Psidium guajava have antidiabetic potential by in vivo for validating the traditional claim of the plant.In this present study we evaluated in vitro alpha amylase and alpha glucosidase activity of crude methanol extract of Psidium guajava leaves. The plant showed significant inhibition activity, so further the compound isolation, purification and characterization which is responsible for inhibiting activity, has to be done for the usage of antidiabetic agent. Acknowledgement The authors are grateful to Dr. P. Selvaraj, Chairman, Shivani Group of Institutions, Trichy, whose spacious heart cheered our efforts to process this venture properly, successfully to complete the task.Also we extend our thanks to The Secretary, The Principal and The Staff members of the SSK Polytechni c College, Trichy, Tamilnadu, India. References Ali, H. , P. J. Houghton, and Soumyanath, A. 2006. J. Ethnopharmacol. 107: 449-455. Evans, W. C. , and Evans, T. 2003. Pharmacognosy, 5th Edn. , Cambridge University Press, London, 336-93. Int. J. Curr. Microbiol. App. Sci (2013) 2(2):15-19 Karthic, K. , K. S. Kirthiram, S. Sadasivam, and Thayumanavan, B. 2008. Identification of amylase inhibitors from Syzygium cumini Linn seeds. Indian J. Exp. Biol. 6:677- 680. Krishnaveni,S. , B.Theymoli, and Sadasivam,S. 1984. Food Chem. 15: 229. Malik,C. P. , and Singh, M. B. 1980. Plant Enzymology and Histoenzymology, Kalyani Publishers, New Delhi, p. 278. Rajiv Gandhi, G. , and Sasikumar, P. 2012. Antidiabetic effect of Merremia emarginata Burm. F. in Streptozotocin induced diabetic rats. Asian. Paci. J. Tropi. Biomedicine. 2: 281-286. Rhabaso Lhoret, R. , and Chiasson, J. L. , 2004. Glucosidase inhibitors. In: Defronzo R. A, Ferrannini E, Keen H, Zimmet P. (Eds. ), International Textbook of Di abetes Mellitus, Vol. 1, third ed. John Wiley and Sons Ltd. , UK, pp. 901-914. Ross, I. A. 1999. Medicinal Plants of the World, Chemical constituents, traditional and modern medicinal uses. Human Press Inc. , Totowa, NJ. 263-272. Shai, L. J. , P. Masoko, M. PMokgotho, S. R. Magano, M. A. Mogale, N. Boaduo, and Eloff, J. N. 2010. South African J. Bot. 76: 465 470. Syamsudin,S. , 2010. Standardization of extract of Leucaena leucocephala (lmk) De Wit seeds by -glucosidase inhibitor. Int. J. Phytomedicine. 2. 430-435. Wild, S. , G. Roglic, A. Green, R. Sicree, and King, H. 2004. Global prevalence of Diabetes estimates for the year 2000 and projections for 2030. Diabetes care. 27:1047-1053. 19